Gelatinous yellowish disco on wet wood

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  • #30239

    Hi,

    I am stuck on this little disco found growing together with what looks like the early stages of Bulbillomyces on a stick in a ditch at Longshaw, early June.

    Numerous discs, yellowish, translucent, drying ochre-brown, sessile, very gelatinous , up to 0.7 mm diameter.

    Spores from squash preparation were approx 8-11 x 3-4, with few small droplets.

    Asci were 75-80 x 5-8, inoperculate, with tips blueing in Lugol.

    Paraphyses seemed to be cylindrical, 2 wide, septate.

    No hairs were seen. Non-hymenial cells were hard to separate, they looked to be roughly columnar, gelatinised and brownish.

    There were some odd-looking brown inclusions in some cells.

    Superficially Paraorbiliopsis minuta looks similar, but that has a well-defined disc edge, large droplets in larger spores and forked paraphyses, and is described as being whitish in colour.

    Any help would be appreciated,

    Cheers,

    Steve

    1 Stereo microscope image x45.JPG

    [attachment=5]1 Stereo microscope image x45.JPG[/attachment]

    2 Asci in Lugol x 1000.jpg

    [attachment=4]2 Asci in Lugol x 1000.jpg[/attachment]

    3 Asci in water  x600.jpg

    [attachment=3]3 Asci in water x600.jpg[/attachment]

    4 Asci and paraphyses in water x600.jpg

    [attachment=2]4 Asci and paraphyses in water x600.jpg[/attachment]

    5 Non hymenial cells in water x600, herbarium specimen .jpg

    [attachment=1]5 Non hymenial cells in water x600, herbarium specimen .jpg[/attachment]

    6 Brown inclusions, in water x600, herbarium specimen.jpg

    [attachment=0]6 Brown inclusions, in water x600, herbarium specimen.jpg[/attachment]

    #38472

    I posted this on Asco France but it proved impossible to ID. I had dried the specimen as a herbarium sample, but this rendered it unsuitable. Looking at dead cells is different from looking at live ones. Moral: keep your ascos in a damp incubator in a cool place until you have an ID.

    Steve

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