Gelatinous yellowish disco on wet wood

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Steve
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Posts: 442
Joined: Sun May 17, 2015 8:59 pm
Location: Sheffield, Yorkshire

Gelatinous yellowish disco on wet wood

Post by Steve » Fri Jul 08, 2016 12:41 pm

Hi,
I am stuck on this little disco found growing together with what looks like the early stages of Bulbillomyces on a stick in a ditch at Longshaw, early June.
Numerous discs, yellowish, translucent, drying ochre-brown, sessile, very gelatinous , up to 0.7 mm diameter.
Spores from squash preparation were approx 8-11 x 3-4, with few small droplets.
Asci were 75-80 x 5-8, inoperculate, with tips blueing in Lugol.
Paraphyses seemed to be cylindrical, 2 wide, septate.
No hairs were seen. Non-hymenial cells were hard to separate, they looked to be roughly columnar, gelatinised and brownish.
There were some odd-looking brown inclusions in some cells.
Superficially Paraorbiliopsis minuta looks similar, but that has a well-defined disc edge, large droplets in larger spores and forked paraphyses, and is described as being whitish in colour.
Any help would be appreciated,
Cheers,
Steve
1 Stereo microscope image x45.JPG
Stereo microscope image x45
2 Asci in Lugol x 1000.jpg
Asci in Lugol x 1000
3 Asci in water  x600.jpg
Asci in Lugol x 600
4 Asci and paraphyses in water x600.jpg
Asci and paraphyses in water x600
5 Non hymenial cells in water x600, herbarium specimen .jpg
Non hymenial cells in water x600, herbarium specimen
6 Brown inclusions, in water x600, herbarium specimen.jpg
Brown inclusions, in water x600, herbarium specimen

Steve
Frequent user
Posts: 442
Joined: Sun May 17, 2015 8:59 pm
Location: Sheffield, Yorkshire

Re: Gelatinous yellowish disco on wet wood

Post by Steve » Tue Jul 12, 2016 10:53 am

I posted this on Asco France but it proved impossible to ID. I had dried the specimen as a herbarium sample, but this rendered it unsuitable. Looking at dead cells is different from looking at live ones. Moral: keep your ascos in a damp incubator in a cool place until you have an ID.
Steve

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