Myxo on dung?

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jimmymac2
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Myxo on dung?

Post by jimmymac2 » Sat Sep 26, 2015 12:57 pm

Found on horse dung near a Pine stand on Ashdown Forest. I would love to do microscopy but I'm not sure how.
RSCN9167.JPG
DSCN9168r.jpg
Always keep your eyes open... :shock:

Waxcap
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Re: Myxo on dung?

Post by Waxcap » Sat Sep 26, 2015 4:23 pm

Hi,

not a Myxomycete but, with that granular outer surface, possibly Cheilymenia granulata, a member of the Ascomycota. You can suspend it over a slide overnight to collect some spores (if you are lucky) see if you can see the striations under oil X100. To see the asci and paraphyses you need to take a very thin section of the hymenial surface and gently squash it on the slide.
http://www.newforest.com/page_2.asp?epi ... &x=70&y=56

Dave

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Re: Myxo on dung?

Post by jimmymac2 » Sat Sep 26, 2015 5:14 pm

Thanks Waxcap
Are these OK? The spores, if they drop, will be coming soon.
Ashdown Forest - Dung-dwelling Asco - Asci001 - Unmeasured.jpg
10x
Ashdown Forest - Dung-dwelling Asco - Asci002.jpg
4x
Ashdown Forest - Dung-dwelling Asco - Asci003 - Apical Pore.jpg
40x
Ashdown Forest - Dung-dwelling Asco - Asci003 - Apical Pore002.jpg
40x
Last edited by jimmymac2 on Sun Sep 27, 2015 11:40 am, edited 3 times in total.
Always keep your eyes open... :shock:

Waxcap
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Re: Myxo on dung?

Post by Waxcap » Sat Sep 26, 2015 6:18 pm

An excellent start. Which objective were you using? I'd suggest asci at 40X and spores at 100X in oil.

Dave

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jimmymac2
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Re: Myxo on dung?

Post by jimmymac2 » Sat Sep 26, 2015 6:45 pm

That's good to hear!
I'm labelling the photos with the objectives used.
Always keep your eyes open... :shock:

Waxcap
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Re: Myxo on dung?

Post by Waxcap » Sun Sep 27, 2015 9:18 am

With Asco's the shape of the paraphyses is important and you can see the inflated ends nicely in your 10x photo. Most asco's have 8 spores in each ascus but there are exceptions. Seeing the spores in the ascus is helpful as you get an idea of the size and shape but to get an accurate size and shape - and see the all important surface ornamentation (or lack of it) - you must look at naturally released spores in something like Cotton Blue stain at 100x, under oil.

Dave

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