Using the oil objective

Discussion about cameras, microscopes, stains, and gadgets, along with useful tips for preparation of fungi samples
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Waxcap
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Using the oil objective

Post by Waxcap » Sun Apr 17, 2016 11:14 am

For those that are new to using a microscope to identify fungi I thought I would post this illustration of the value of using the 100x oil objective and a dash of cotton Blue for seeing spore ornamentation.

I was looking at Aspergillus niger (on mouldy bread) and thought I might get away with using my dry 60X objective to look at the spores. It's a Plan APO objective and is very good quality. Here are the results.

Spores under 60X dry objective
Bread_Mould-2.jpg
I couldn't find a Aspergillus with that size smooth spores so I thought I would have a look with the 100x oil objective.

The same spores under 100X oil objective.
Aspergillus.jpg
Again with the 100X oil but here there is a slight lensing effect and contrast increase as the spores were trapped in a bubble of air.
Aspergillus_niger_spores4.jpg
Using the 100x objective is also good for seeing the spore wall thickness and faint germ pores.

Dave

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adampembs
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Re: Using the oil objective

Post by adampembs » Sat Jun 18, 2016 9:26 pm

Interesting to see the difference :)
Adam Pollard
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