Bolete ID Please! :)

Please try to include photos to show all parts of the fungus, eg top, stem, and gills.
Note any smells, and associated trees or plants (eg oak, birch). A spore print can be very useful.
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Lancashire Lad
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Re: Bolete ID Please! :)

Post by Lancashire Lad » Tue Sep 01, 2015 7:20 pm

Hi Jimmy,

Your photos show that you've basically done the calibrations for each lens correctly - but not quite accurately enough!

The first photo (x40) shows the full 1000µ of the graticule's length, but you have measured only 100µ.
To see how accurate your calibration actually is, you should draw the measurement line as long as possible.
So in this case you'd get a better idea of how accurate your calibration was by drawing the measurement line across the full 1000µ length.

The second photo (x100) looks pretty accurate, but again, you would get a better idea of just how accurate by drawing the measurement line across, say 400µ, instead of just 100µ.

The third photograph (x400) shows that you have a slightly wrong calibration. (certainly for that particular objective)
The measurement says 100µ, but the line starts at the left hand side of a segment line, and finishes at the right hand side of one.
Therefore, you have measured 100µ plus the width of a line - so in actual fact your measurement here should really be saying about 103-104µ
3 or 4% inaccuracy might not sound like a lot, but it can be important when measuring things as small as spores.
To avoid that type of inaccuracy, always finish the calibration measurement line at the same side of a graticule marker line as the one where you started the line.
(I always calibrate from left edge of one marker line to left edge of another marker line).

Just to reconfirm - when you are actually doing the calibration itself, always draw the measurement line across as many identifiable segments as it is possible to determine within the field of view.
(e.g. if you can see 430µ of the graticule, do the calibration line across 400µ. you will get a more accurate result than if you do the calibration line across just 100 or 200µ.

PS - don't miss out the oil immersion lens from your calibration strategy - you will need to use that lens a great deal if you want to get the best information from spores in general.
OK - you will have to put a drop of immersion oil onto your graticule in order to be able to calibrate the oil immersion lens, but (provided you do the calibration accurately ;) ) you will only need to do it once, and you should be able to easily (carefully) clean the graticule afterwards by wiping it with soft tissue.

Hope that helps.

Regards,
Mike.
Common sense is not so common.

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Chris Yeates
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Re: Bolete ID Please! :)

Post by Chris Yeates » Tue Sep 01, 2015 8:20 pm

congrat's to Mike for all that effort - here and elsewhere . . . hope it's appreciated
C
"You must know it's right, the spore is on the wind tonight"
Steely Dan - "Rose Darling"

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adampembs
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Re: Bolete ID Please! :)

Post by adampembs » Tue Sep 01, 2015 10:43 pm

Lancashire Lad wrote:
PS - don't miss out the oil immersion lens from your calibration strategy - you will need to use that lens a great deal if you want to get the best information from spores in general.


Regards,
Mike.
Actually Jimmy, this is THE most important one to measure as you should ALWAYS measure small spores like basidiospores with the x100 lens as the error margin in measuring small spores at x40 is much greater than x100. It's not so bad with big spores such as boletes (or rust spores), but would be much more noticeable with 3-4µm long spores.
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jimmymac2
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Re: Bolete ID Please! :)

Post by jimmymac2 » Wed Sep 02, 2015 5:26 am

Thanks for your comments. I'll redo the 400x on the weekend :roll: and the 1000x as well.
No comment on the actual bolete though??
Always keep your eyes open... :shock:

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Re: Bolete ID Please! :)

Post by adampembs » Wed Sep 02, 2015 8:18 am

jimmymac2 wrote: No comment on the actual bolete though??
You won't get a better answer than the one Andreas already gave you.
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