Fungus on Sonchus.asper - help to ID needed please

Please try to include photos to show all parts of the fungus, eg top, stem, and gills.
Note any smells, and associated trees or plants (eg oak, birch). A spore print can be very useful.
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Please do not ask for the identification of fungi for edibility or narcotic purposes. Any help provided by forum members is on the understanding that fungi are not to be consumed. Any deaths or serious poisonings are the responsibility of the person eating or preparing the fungus for others. If it is apparent from a post that the fungus is for eating or smoking etc, the post will be deleted and a warning given. Although many members do eat fungi, no-one would be willing to take someone else's life into their hands.
JohnWB
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Re: Fungus on Sonchus.asper - help to ID needed please

Post by JohnWB » Thu Apr 12, 2018 8:51 pm

Hi all, I've cut and mounted some thinner sections of 5µ (the earlier images are of 8µ sections) and changed the staining to try to make finer details more visible.

Here are a few views of what I think are hyphae having entered the unfortunate Sonchus.arvensis via stomata.
The hypha internal image seems to show the hypha making it's way through the cortex of the stem using the intercellular spaces rather than penetrating cell-walls to move directly through cells. In the image it has arrived at the vascular-bundle fiber-cap - beyond which in the direction travelled, lie the phloem sugar-conducting vessels - could this be the target for the hypha to take nutrient from the Sonchus food-transport directly?

The more I see of these 'micro-fungi' on plants the more interesting they become - including the different stages - up to 5 in Miyagia.pseudosphaeria (the probable identity if this 'Sonchus fungus' it seems.
Attachments
ws_sonchus arvensis fungus (1).jpg
ws_sonchus arvensis fungus (3).jpg
ws_sonchus arvensis fungus (4).jpg
ws_sonchus arvensis fungus (6).jpg

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Lancashire Lad
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Re: Fungus on Sonchus.asper - help to ID needed please

Post by Lancashire Lad » Fri Apr 13, 2018 12:36 pm

Hi John,

Thanks for bearing with us re the current "multi image" upload problem. - I'm pleased that you are managing the workaround effectively!

And thanks again for your updates on this topic, and your truly excellent photos.

Your microscopy techniques of sectioning/staining/photography are as good as I've ever seen.
If you would be willing to give a description of the techniques you are using, (perhaps as a new topic in our "Microscopes, Cameras, Equipment, & Technology" forum, I'm absolutely certain that many of UK Fungi's members would find such an insight very interesting indeed!

I can't claim to be much more than a beginner with rust fungi (and with any other fungi come to that. :D :oops: ).
Chris Yeates is THE expert in that field, and I'm sure he will comment on your images when he sees them.

I think I'm correct in saying that all rust fungi obtain their nutrients from the host plant by way of specialised cells named "haustoria" which they produce once they've penetrated into the host's leaf or stem.

Perhaps worth looking for, as with your microscopy skills and sectioning technique, I'm sure that you would be able to get some outstanding images!

In case it's of interest to you, here are a couple of links to articles that give an overview of what goes on when a rust fungus attacks a plant: -

http://journals.plos.org/plospathogens/ ... at.1004329

http://www.pnas.org/content/98/14/7654

Regards,
Mike.
Common sense is not so common.

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Re: Fungus on Sonchus.asper - help to ID needed please

Post by JohnWB » Sun Apr 15, 2018 2:24 pm

Hi again, I've a few more images from some 8µ and 5µ slides this time, just finished them yesterday...
The first image may be a germinating spore with emergent hypha? I'm hoping someone will be able to help me out here, I know virtually nothing about fungi, but certainly plan to study them more as I find them very interesting indeed, and they make nice slides too!
The rest are a selection all of the sectioned Sonchus.arvensis with the Miyagia.pseudosphaeria infection. I suspect several 'stages' are present in and on the plant, but I don't have the expertise to recognize them - although I think I've seen bi-nucleate teliospores before nuclear-fusion. not sure though...

Sorry to just 'dump' the images but with the problems at present with images here it's the only way I can get them to persist rather than vanish - hope they all show-up....
Here are some more images,
Attachments
14_04_18_438_stitch.jpg
14_04_18_442.JPG
fungus outside epidermis.jpg
leaf vasc bundle_edited-1.jpg
ps edited stack3.jpg
spore maybe.JPG
stack1.jpg
stacket spore.jpg

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